A PARAFFIN EMBEDDING TECHNIQUE FOR STUDIES EMPLOYING IMMUNOFLUORESCENCE

Paraffin embedding technique for specimens obtained by vitrectomy.

Histologic and immunohistochemical preparation of pathologic fluids containing cellular material is difficult, because the techniques are limited by the amount of cellular material. Similar problems limit the preparation of vitrectomized material. Cytopreparatory techniques, including millipore filter and celloidin bag cell block, have been introduced since 1980 for diagnostic vitrectomies. The...

Multiparameter immunofluorescence on paraffin-embedded tissue sections.

Immunohistochemical techniques have gained increasing importance in diagnostics and research. While formalin-fixed, paraffin-embedded human tissue retains excellent morphology, the detection of antigens by immunofluorescence in its sections and especially the demonstration of multiple simultaneous antibodies have limitations. Double immunofluorescence labeling of routinely processed paraffin se...

A Rapid Method of Paraffin Embedding

Paraffin immunofluorescence for detection of immune complexes in renal biopsies: an efficient salvage technique for diagnosis of glomerulonephritis in dogs

BACKGROUND Renal biopsy is an essential tool for the diagnosis of proteinuric kidney diseases in dogs, and evaluation of immune complexes (IC) by immunofluorescence (IF) of frozen sections (IF-F) is required for the diagnosis of IC-mediated glomerulonephritis (ICGN). However, the use of frozen sections from renal biopsies can have limitations. The aim of this study was to develop a reliable IF ...

Agar-gelatin for embedding tissues prior to paraffin processing.

Benchmarks Fixed tissues often need to be arranged in a particular configuration , order, or orientation prior to processing for paraffin embedding. Although it is possible to arrange tissues after infiltration (during the final stages of paraffin embedding), it can be more convenient and practical to do this at the time of dissection or tissue collection. Agar has been the classical medium use...